Gene, Cell and Tissue

Published by: Kowsar

Stable and Transient Expression of Human Coagulation Factor IX in Mammalian Expression Systems; CHO Versus HEK Cells

Jafar Vatandoost 1 , * and Behnaz Dolatabadi 2
Authors Information
1 Department of Biology, Hakim Sabzevari University, Sabzevar, Iran
2 Department of Biotechnology, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran
Article information
  • Gene, Cell and Tissue: April 2017, 4 (2); e13096
  • Published Online: April 30, 2017
  • Article Type: Research Article
  • Received: December 14, 2016
  • Revised: January 29, 2017
  • Accepted: March 30, 2017
  • DOI: 10.5812/gct.13096

To Cite: Vatandoost J, Dolatabadi B. Stable and Transient Expression of Human Coagulation Factor IX in Mammalian Expression Systems; CHO Versus HEK Cells, Gene Cell Tissue. 2017 ; 4(2):e13096. doi: 10.5812/gct.13096.

Abstract
Copyright © 2017, Zahedan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
Acknowledgements
Footnote
References
  • 1. Kim KR, Kim YK, Cha HJ. Recombinant baculovirus-based multiple protein expression platform for Drosophila S2 cell culture. J Biotechnol. 2008; 133(1): 116-22[DOI][PubMed]
  • 2. Khan KH. Gene expression in Mammalian cells and its applications. Adv Pharm Bull. 2013; 3(2): 257-63[DOI][PubMed]
  • 3. Yin J, Li G, Ren X, Herrler G. Select what you need: a comparative evaluation of the advantages and limitations of frequently used expression systems for foreign genes. J Biotechnol. 2007; 127(3): 335-47[DOI][PubMed]
  • 4. Geisse S, Voedisch B. Transient expression technologies: past, present, and future. Methods Mol Biol. 2012; 899: 203-19[DOI][PubMed]
  • 5. Thomas P, Smart TG. HEK293 cell line: a vehicle for the expression of recombinant proteins. J Pharmacol Toxicol Methods. 2005; 51(3): 187-200[DOI][PubMed]
  • 6. Liu C, Dalby B, Chen W, Kilzer JM, Chiou HC. Transient transfection factors for high-level recombinant protein production in suspension cultured mammalian cells. Mol Biotechnol. 2008; 39(2): 141-53[DOI][PubMed]
  • 7. Suen KF, Turner MS, Gao F, Liu B, Althage A, Slavin A, et al. Transient expression of an IL-23R extracellular domain Fc fusion protein in CHO vs. HEK cells results in improved plasma exposure. Protein Expr Purif. 2010; 71(1): 96-102[DOI][PubMed]
  • 8. Goncalves C, Gross F, Guegan P, Cheradame H, Midou P. A robust transfection reagent for the transfection of CHO and HEK293 cells and production of recombinant proteins and lentiviral particles - PTG1. Biotechnol J. 2014; 9(11): 1380-8[DOI][PubMed]
  • 9. Krista S, James B, Meg D, Karen D. Literature Review: CHO versus HEK Cell Glycosylation. 2015;
  • 10. Bohm E, Dockal M, Graninger M, Hasslacher M, Kaliwoda M, Konetschny C, et al. Expression of recombinant human coagulation factors VII (rFVII) and IX (rFIX) in various cell types, glycosylation analysis, and pharmacokinetic comparison. BMC Proc. 2011; 5 Suppl 8[DOI][PubMed]
  • 11. Diepenbruck C, Klinger M, Urbig T, Baeuerle P, Neef R. Productivity and quality of recombinant proteins produced by stable CHO cell clones can be predicted by transient expression in HEK cells. Mol Biotechnol. 2013; 54(2): 497-503[DOI][PubMed]
  • 12. Durocher Y, Butler M. Expression systems for therapeutic glycoprotein production. Curr Opin Biotechnol. 2009; 20(6): 700-7[DOI][PubMed]
  • 13. Vatandoost J, Zomorodipour A, Sadeghizadeh M, Aliyari R, Bos MH, Ataei F. Expression of biologically active human clotting factor IX in Drosophila S2 cells: gamma-carboxylation of a human vitamin K-dependent protein by the insect enzyme. Biotechnol Prog. 2012; 28(1): 45-51[DOI][PubMed]
  • 14. Vatandoost J, Bos MH. Efficient expression of functional human coagulation factor IX in stably-transfected Drosophila melanogaster S2 cells; comparison with the mammalian CHO system. Biotechnol Lett. 2016; 38(10): 1691-8[DOI][PubMed]
  • 15. Jallat S, Perraud F, Dalemans W, Balland A, Dieterle A, Faure T, et al. Characterization of recombinant human factor IX expressed in transgenic mice and in derived trans-immortalized hepatic cell lines. EMBO J. 1990; 9(10): 3295-301[PubMed]
  • 16. Kim YK, Shin HS, Tomiya N, Lee YC, Betenbaugh MJ, Cha HJ. Production and N-glycan analysis of secreted human erythropoietin glycoprotein in stably transfected Drosophila S2 cells. Biotechnol Bioeng. 2005; 92(4): 452-61[DOI][PubMed]
  • 17. Preuss AK, Connor JA, Vogel H. Transient transfection induces different intracellular calcium signaling in CHO K1 versus HEK 293 cells. Cytotechnology. 2000; 33(1-3): 139-45[DOI][PubMed]
  • 18. Jager V, Bussow K, Wagner A, Weber S, Hust M, Frenzel A, et al. High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells. BMC Biotechnol. 2013; 13: 52[DOI][PubMed]
  • 19. Tienen LM. Transient gene expression in mammalian cells for the production of recombinant protein therapeutics. 2012;
  • 20. Sarah W. Transient recombinant protein expression in mammalian cells: The role of mrna level and stability. 2009;
  • 21. Tie JK, Zheng MY, Pope RM, Straight DL, Stafford DW. Identification of the N-linked glycosylation sites of vitamin K-dependent carboxylase and effect of glycosylation on carboxylase function. Biochemistry. 2006; 45(49): 14755-63[DOI][PubMed]
  • 22. Li R. Transient transfection of CHO cells using linear polyethylenimine is a simple and effective means of producing rainbow trout recombinant IFN-gamma protein. Cytotechnology. 2015; 67(6): 987-93[DOI][PubMed]
  • 23. Tienen LM. Transiant gene expression in mammilian cells for the production of recombinant protein therapeutics. 2012;
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