Gene, Cell and Tissue

Published by: Kowsar

In Vitro Evaluation of Cytotoxic and Antiproliferative Effects of Portulaca oleracea Ethanolic Extracton on HeLa Cell Line

Shiva Khatibi 1 , Zahra Farzaneh Taban 1 and Amaneh Mohammadi Roushandeh 2 , 3 , *
Authors Information
1 Biology Department, Basic Sciences faculty, Islamic Azad University of Hamadan, Hamadan, IR Iran
2 Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, IR Iran
3 Department of Anatomical Sciences, School of Medicine, Hamadan University of Medical Sciences, Hamadan, IR Iran
Article information
  • Gene, Cell and Tissue: January 2017, 4 (1); e13301
  • Published Online: November 16, 2016
  • Article Type: Research Article
  • Received: August 17, 2016
  • Revised: November 11, 2016
  • Accepted: November 12, 2016
  • DOI: 10.17795/gct.41565

To Cite: Khatibi S, Farzaneh Taban Z, Mohammadi Roushandeh A. In Vitro Evaluation of Cytotoxic and Antiproliferative Effects of Portulaca oleracea Ethanolic Extracton on HeLa Cell Line, Gene Cell Tissue. 2017 ; 4(1):e13301. doi: 10.17795/gct.41565.

Abstract
Copyright © 2016, Zahedan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
Acknowledgements
Footnotes
References
  • 1. Lutgens L, van der Zee J, Pijls-Johannesma M, De Haas-Kock DF, Buijsen J, Mastrigt GA, et al. Combined use of hyperthermia and radiation therapy for treating locally advanced cervix carcinoma. Cochrane Database Syst Rev. 2010; (3)[DOI][PubMed]
  • 2. Parkin DM, Almonte M, Bruni L, Clifford G, Curado MP, Pineros M. Burden and trends of type-specific human papillomavirus infections and related diseases in the latin america and Caribbean region. Vaccine. 2008; 26 Suppl 11-15[DOI][PubMed]
  • 3. Mitchell RS, Kumar V, Abbas AK, Fausto N. Robbins basic pathology. 2007;
  • 4. Follen M, Meyskens FJ, Alvarez RD, Walker JL, Bell MC, Storthz KA, et al. Cervical cancer chemoprevention, vaccines, and surrogate endpoint biomarkers. Cancer. 2003; 98(9 Suppl): 2044-51[DOI][PubMed]
  • 5. Schlattl A, Anders S, Waszak SM, Huber W, Korbel JO. Relating CNVs to transcriptome data at fine resolution: assessment of the effect of variant size, type, and overlap with functional regions. Genome Res. 2011; 21(12): 2004-13[DOI][PubMed]
  • 6. Culliton BJ. HeLa Cells: Contaminating Cultures around the World. Science. 1974; 184(4141): 1058-9[DOI][PubMed]
  • 7. Puck TT, Marcus PI. A Rapid Method for Viable Cell Titration and Clone Production with Hela Cells in Tissue Culture: The Use of X-Irradiated Cells to Supply Conditioning Factors. Proc Natl Acad Sci U S A. 1955; 41(7): 432-7[PubMed]
  • 8. Defendi V, Billingham RE, Silvers WK, Moorhead P. Immunological and karyological criteria for identification of cell lines. J Natl Cancer Inst. 1960; 25: 359-85[PubMed]
  • 9. Gartler SM. Apparent Hela cell contamination of human heteroploid cell lines. Nature. 1968; 217(5130): 750-1[PubMed]
  • 10. Pandey A, Tripathi S. Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug. J Pharm Phytochem. 2014; 2(5)
  • 11. Rashed AN, Afifi FU, Disi AM. Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea L. (growing in Jordan) in Mus musculus JVI-1. J Ethnopharmacol. 2003; 88(2-3): 131-6[PubMed]
  • 12. Lim YY, Quah EPL. Antioxidant properties of different cultivars of Portulaca oleracea. Food Chem. 2007; 103(3): 734-40[DOI]
  • 13. Aberoumand A. Nutritional Evaluation of Edible Portulaca oleracia as Plant Food. Food Analyt Methods. 2008; 2(3): 204-7[DOI]
  • 14. Malek F, Boskabady MH, Borushaki MT, Tohidi M. Bronchodilatory effect of Portulaca oleracea in airways of asthmatic patients. J Ethnopharmacol. 2004; 93(1): 57-62[DOI][PubMed]
  • 15. Radhakrishnan R, Zakaria MN, Islam MW, Chen HB, Kamil M, Chan K, et al. Neuropharmacological actions of Portulaca oleraceae L v. sativa (Hawk). J Ethnopharmacol. 2001; 76(2): 171-6[PubMed]
  • 16. Kim CH, Park PB, Choe SR, Kimz TH, Lee JKJKG, Lee CH, et al. Anti-oxidative and Anti-inflammatory Effects of Protulaca O/eracea on the LPS-stimulated AGS Cells. Korean J Orient Physiol Pathol. 2009; 23(2)-93
  • 17. Islam MW, Zakaria MNM, Radhakrishnan R, Habibullah M, Chan K. Evaluation of analgesic activity of the aerial parts of Portulaca oleracea V. sativa and its comparison with two related species. J Pharm Pharmacol. 1998; 50: 226[DOI]
  • 18. Xiang L, Xing D, Wang W, Wang R, Ding Y, Du L. Alkaloids from Portulaca oleracea L. Phytochemistry. 2005; 66(21): 2595-601[DOI][PubMed]
  • 19. Sudhakar D, Krishna Kishore R, Parthasarathy PR. Portulaca oleracea L. extract ameliorates the cisplatin-induced toxicity in chick embryonic liver. Indian J Biochem Biophys. 2010; 47(3): 185-9[PubMed]
  • 20. Karimi G, Aghasizadeh M, Razavi M, Taghiabadi E. Protective effects of aqueous and ethanolic extracts of Nigella sativa L. and Portulaca oleracea L. on free radical induced hemolysis of RBCs. Daru. 2011; 19(4): 295-300[PubMed]
  • 21. Cai Y, Luo Q, Sun M, Corke H. Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 2004; 74(17): 2157-84[DOI][PubMed]
  • 22. Simopoulos AP. Omega-3 fatty acids and antioxidants in edible wild plants. Biol Res. 2004; 37(2): 263-77[PubMed]
  • 23. Azarifar Z, Mortazavi M, Farhadian R, Parvari S, Mohammadi Roushandeh A. Cytotoxicity Effects of Aqueous Extract of Purtulaca oleracea on HeLa cell Line. Pharm Sci. 2015; 21(1): 41-5[DOI]
  • 24. Payudara S, Dan Nasofarinks K, Tan G, Wong K, PEArLE-WoNG GQ, Yeo SL, et al. In vitro Cytotoxic and antiproliferative effects of Portulaca oleracea methanol extract on breast, cervical, colon and nasopharyngeal cancerous cell lines. Sains Malays. 2013; 42: 927-35
  • 25. As Z, Jh H. Cytogenetic toxicity effects of local purslane (portulaca oleracea) leaf crude extracts on normal and cancer cell lines in vitro. Int J Drug Discover. 2013; 5(1): 173-80[DOI]
  • 26. Hassan A. Chemical and remedial effects of purslane (portulaca oleracea) plant. Life Sci J. 2014; 11: 31-42
  • 27. Lepley DM, Li B, Birt DF, Pelling JC. The chemopreventive flavonoid apigenin induces G2/M arrest in keratinocytes. Carcinogenesis. 1996; 17(11): 2367-75[PubMed]
  • 28. Al-Sheddi ES, Farshori NN, Al-Oqail MM, Musarrat J, Al-Khedhairy AA, Siddiqui MA. Portulaca oleracea Seed Oil Exerts Cytotoxic Effects on Human Liver Cancer (HepG2) and Human Lung Cancer (A-549) Cell Lines. Asian Pac J Cancer Prev. 2015; 16(8): 3383-7[PubMed]
  • 29. Liang YC, Huang YT, Tsai SH, Lin-Shiau SY, Chen CF, Lin JK. Suppression of inducible cyclooxygenase and inducible nitric oxide synthase by apigenin and related flavonoids in mouse macrophages. Carcinogenesis. 1999; 20(10): 1945-52[PubMed]
  • 30. Staud R. Comparing the COX-2 Inhibitors with Traditional NSAIDs. Emerg Med J. 2000; : 13-22
  • 31. Lim DY, Cho HJ, Kim J, Nho CW, Lee KW, Park JH. Luteolin decreases IGF-II production and downregulates insulin-like growth factor-I receptor signaling in HT-29 human colon cancer cells. BMC Gastroenterol. 2012; 12: 9[DOI][PubMed]
  • 32. Anderson N, Lokich JJ. Cancer chemotherapy and infusional scheduling. Oncology. 1994; 8(5): 99-111
  • 33. Basu S, Nagy JA, Pal S, Vasile E, Eckelhoefer IA, Bliss VS, et al. The neurotransmitter dopamine inhibits angiogenesis induced by vascular permeability factor/vascular endothelial growth factor. Nat Med. 2001; 7(5): 569-74[DOI][PubMed]
  • 34. Chen T, Wang J, Li Y, Shen J, Zhao T, Zhang H. Sulfated modification and cytotoxicity of Portulaca oleracea L. polysaccharides. Glycoconj J. 2010; 27(6): 635-42[DOI][PubMed]
  • 35. Wang TS, Chen LJ, Wang ZY, Zhang ST, Lin JM. Purified alkaloid extract of Scutellaria barbata inhibits proliferation of hepatoma HepG-2 cells by inducing apoptosis and cell cycle arrest at G2/M phase. Afr J Pharm Pharmacol. 2011; 5(8): 1046-53
  • 36. Zhao R, Gao X, Cai Y, Shao X, Jia G, Huang Y, et al. Antitumor activity of Portulaca oleracea L. polysaccharides against cervical carcinoma in vitro and in vivo. Carbohydr Polym. 2013; 96(2): 376-83[DOI][PubMed]
  • 37. Zhao R, Zhang T, Zhao H, Cai Y. Effects of Portulaca oleracea L. Polysaccharides on Phenotypic and Functional Maturation of Murine Bone Marrow Derived Dendritic Cells. Nutr Cancer. 2015; 67(6): 987-93[DOI][PubMed]
  • 38. Lehner B, Sandner B, Marschallinger J, Lehner C, Furtner T, Couillard-Despres S, et al. The dark side of BrdU in neural stem cell biology: detrimental effects on cell cycle, differentiation and survival. Cell Tissue Res. 2011; 345(3): 313-28[DOI][PubMed]
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