Gene, Cell and Tissue

Published by: Kowsar

Promoter Methylation and mRNA Expression of APAF-1 Gene in Breast Cancer

Ebrahim Eskandari-Nasab 1 , 2 and Mohammad Hashemi 2 , *
Authors Information
1 Genetic of Non-Communicable Disease Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran
2 Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IR Iran
Article information
  • Gene, Cell and Tissue: January 2017, 4 (1); e13332
  • Published Online: December 18, 2016
  • Article Type: Research Article
  • Received: November 12, 2016
  • Revised: December 4, 2016
  • Accepted: December 5, 2016
  • DOI: 10.17795/gct-43748

To Cite: Eskandari-Nasab E, Hashemi M. Promoter Methylation and mRNA Expression of APAF-1 Gene in Breast Cancer, Gene Cell Tissue. 2017 ; 4(1):e13332. doi: 10.17795/gct-43748.

Copyright © 2016, Zahedan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Introduction
2. Methods
3. Results
4. Discussion
  • 1. Zubair A, Frieri M. Role of nuclear factor-kB in breast and colorectal cancer. Curr Allergy Asthma Rep. 2013; 13(1): 44-9[DOI][PubMed]
  • 2. Kobayashi S, Sugiura H, Ando Y, Shiraki N, Yanagi T, Yamashita H, et al. Reproductive history and breast cancer risk. Breast Cancer. 2012; 19(4): 302-8[DOI][PubMed]
  • 3. Esteller M. Relevance of DNA methylation in the management of cancer. Lancet Oncol. 2003; 4(6): 351-8[PubMed]
  • 4. Gopisetty G, Ramachandran K, Singal R. DNA methylation and apoptosis. Mol Immunol. 2006; 43(11): 1729-40[DOI][PubMed]
  • 5. Esteller M. Epigenetic lesions causing genetic lesions in human cancer: promoter hypermethylation of DNA repair genes. Eur J Cancer. 2000; 36(18): 2294-300[PubMed]
  • 6. Le Romancer M, Treilleux I, Bouchekioua-Bouzaghou K, Sentis S, Corbo L. Methylation, a key step for nongenomic estrogen signaling in breast tumors. Steroids. 2010; 75(8-9): 560-4[DOI][PubMed]
  • 7. Grau E, Martinez F, Orellana C, Canete A, Yanez Y, Oltra S, et al. Hypermethylation of apoptotic genes as independent prognostic factor in neuroblastoma disease. Mol Carcinog. 2011; 50(3): 153-62[DOI][PubMed]
  • 8. Zhu X, Yi F, Chen P, Chen L, Zhang X, Cao C, et al. 5-Aza-2'-Deoxycytidine and CDDP Synergistically Induce Apoptosis in Renal Carcinoma Cells via Enhancing the APAF-1 Activity. Clin Lab. 2015; 61(12): 1821-30[PubMed]
  • 9. Roman-Gomez J, Jimenez-Velasco A, Castillejo JA, Agirre X, Barrios M, Navarro G, et al. Promoter hypermethylation of cancer-related genes: a strong independent prognostic factor in acute lymphoblastic leukemia. Blood. 2004; 104(8): 2492-8[DOI][PubMed]
  • 10. Soengas MS, Capodieci P, Polsky D, Mora J, Esteller M, Opitz-Araya X, et al. Inactivation of the apoptosis effector Apaf-1 in malignant melanoma. Nature. 2001; 409(6817): 207-11[DOI][PubMed]
  • 11. Zlobec I, Minoo P, Baker K, Haegert D, Khetani K, Tornillo L, et al. Loss of APAF-1 expression is associated with tumour progression and adverse prognosis in colorectal cancer. Eur J Cancer. 2007; 43(6): 1101-7[DOI][PubMed]
  • 12. Ahmad ST, Arjumand W, Seth A, Saini AK, Sultana S. Methylation of the APAF-1 and DAPK-1 promoter region correlates with progression of renal cell carcinoma in North Indian population. Tumour Biol. 2012; 33(2): 395-402[DOI][PubMed]
  • 13. Christoph F, Kempkensteffen C, Weikert S, Kollermann J, Krause H, Miller K, et al. Methylation of tumour suppressor genes APAF-1 and DAPK-1 and in vitro effects of demethylating agents in bladder and kidney cancer. Br J Cancer. 2006; 95(12): 1701-7[DOI][PubMed]
  • 14. Furukawa Y, Sutheesophon K, Wada T, Nishimura M, Saito Y, Ishii H, et al. Methylation silencing of the Apaf-1 gene in acute leukemia. Mol Cancer Res. 2005; 3(6): 325-34[DOI][PubMed]
  • 15. Li C, Wang L, Su J, Zhang R, Fu L, Zhou Y. mRNA expression and hypermethylation of tumor suppressor genes apoptosis protease activating factor-1 and death-associated protein kinase in oral squamous cell carcinoma. Oncol Lett. 2013; 6(1): 280-6[DOI][PubMed]
  • 16. Kordi-Tamandani DM, Ladies MA, Hashemi M, Moazeni-Roodi AK, Krishna S, Torkamanzehi A. Analysis of p15INK4b and p16INK4a gene methylation in patients with oral squamous cell carcinoma. Biochem Genet. 2012; 50(5-6): 448-53[DOI][PubMed]
  • 17. Eskandari-Nasab E, Hashemi M, Amininia S, Ebrahimi M, Rezaei M, Hashemi SM. Effect of TP53 16-bp and beta-TrCP 9-bp INS/DEL polymorphisms in relation to risk of breast cancer. Gene. 2015; 568(2): 181-5[DOI][PubMed]
  • 18. Eskandari-Nasab E, Hashemi M, Rafighdoost F. Promoter Methylation and mRNA Expression of Response Gene to Complement 32 in Breast Carcinoma. J Cancer Epidemiol. 2016; 2016: 7680523[DOI][PubMed]
  • 19. Hashemi M, Rezaei H, Eskandari-Nasab E, Kaykhaei MA, Taheri M. Association of promoter methylation and 32-bp deletion of the PTEN gene with susceptibility to metabolic syndrome. Mol Med Rep. 2013; 7(1): 342-6[DOI][PubMed]
  • 20. Yuan JS, Reed A, Chen F, Stewart CJ. Statistical analysis of real-time PCR data. BMC Bioinformatics. 2006; 7: 85[DOI][PubMed]
  • 21. Poplawski T, Tomaszewska K, Galicki M, Morawiec Z, Blasiak J. Promoter methylation of cancer-related genes in gastric carcinoma. Exp Oncol. 2008; 30(2): 112-6[PubMed]
  • 22. Teodoridis JM, Hall J, Marsh S, Kannall HD, Smyth C, Curto J, et al. CpG island methylation of DNA damage response genes in advanced ovarian cancer. Cancer Res. 2005; 65(19): 8961-7[DOI][PubMed]
  • 23. Chim CS, Liang R, Fung TK, Choi CL, Kwong YL. Epigenetic dysregulation of the death-associated protein kinase/p14/HDM2/p53/Apaf-1 apoptosis pathway in multiple myeloma. J Clin Pathol. 2007; 60(6): 664-9[DOI][PubMed]
  • 24. Levine AJ, Momand J, Finlay CA. The p53 tumour suppressor gene. Nature. 1991; 351(6326): 453-6[DOI][PubMed]
  • 25. Yasui W, Oue N, Aung PP, Matsumura S, Shutoh M, Nakayama H. Molecular-pathological prognostic factors of gastric cancer: a review. Gastric Cancer. 2005; 8(2): 86-94[DOI][PubMed]
  • 26. Xing D, Tan W, Lin D. Genetic polymorphisms and susceptibility to esophageal cancer among Chinese population (review). Oncol Rep. 2003; 10(5): 1615-23[PubMed]
  • 27. Baliga B, Kumar S. Apaf-1/cytochrome c apoptosome: an essential initiator of caspase activation or just a sideshow? Cell Death Differ. 2003; 10(1): 16-8[DOI][PubMed]
  • 28. Yoshida H. The role of Apaf-1 in programmed cell death: from worm to tumor. Cell Struct Funct. 2003; 28(1): 3-9[PubMed]
  • 29. Zermati Y, Mouhamad S, Stergiou L, Besse B, Galluzzi L, Boehrer S, et al. Nonapoptotic role for Apaf-1 in the DNA damage checkpoint. Mol Cell. 2007; 28(4): 624-37[DOI][PubMed]
  • 30. Campioni M, Santini D, Tonini G, Murace R, Dragonetti E, Spugnini EP, et al. Role of Apaf-1, a key regulator of apoptosis, in melanoma progression and chemoresistance. Exp Dermatol. 2005; 14(11): 811-8[DOI][PubMed]
  • 31. Ji L, Roth JA. Tumor suppressor FUS1 signaling pathway. J Thorac Oncol. 2008; 3(4): 327-30[DOI][PubMed]
  • 32. Xiong H, Qiu H, Zhuang L, Xiong H, Jiang R, Chen Y. Effects of 5-Aza-CdR on the proliferation of human breast cancer cell line MCF-7 and on the expression of Apaf-1 gene. J Huazhong Univ Sci Technolog Med Sci. 2009; 29(4): 498-502[DOI][PubMed]
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