Gene, Cell and Tissue

Published by: Kowsar

Digoxin Effectively Inhibited Cell Growth and Induced Senescence in Cervical Cancer Cell Line

Atefeh Tahervand 1 , Behnaz Parnian 1 , Mehryar Habibi Roudkenar 2 and Amaneh Mohammadi Roushandeh 3 , *
Authors Information
1 Biology Department, Basic Sciences faculty, Islamic Azad University of Hamadan, Hamadan, Iran
2 Medical Biotechnology Research Center, Faculty of Paramedicine, Guilan University of Medical Sciences, Rasht, Iran
3 Anatomical Sciences Department, Medicine Faculty, Hamadan University of Medical Sciences, Hamadan, Iran
Article information
  • Gene, Cell and Tissue: April 2017, 4 (2); e14216
  • Published Online: April 30, 2017
  • Article Type: Research Article
  • Received: February 1, 2017
  • Revised: March 4, 2017
  • Accepted: March 28, 2017
  • DOI: 10.5812/gct.14216

To Cite: Tahervand A, Parnian B, Habibi Roudkenar M, Mohammadi Roushandeh A. Digoxin Effectively Inhibited Cell Growth and Induced Senescence in Cervical Cancer Cell Line, Gene Cell Tissue. 2017 ; 4(2):e14216. doi: 10.5812/gct.14216.

Copyright © 2017, Gene, Cell and Tissue. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Khaizil Emylia Z, Nik Aina SNZ, Mohd Dasuki S. Preliminary study on anti-proliferative activity of methanolic extract of nephelium lappaceum peels towards breast (MDA-MB-231), cervical (HeLa) and osteosarcoma (MG-63) cancer cell lines. Health. 2013; 4(2): 66-79
  • 2. Sanda MG, Dunn RL, Michalski J, Sandler HM, Northouse L, Hembroff L, et al. Quality of life and satisfaction with outcome among prostate-cancer survivors. N Engl J Med. 2008; 358(12): 1250-61[DOI][PubMed]
  • 3. Lin H, Juang JL, Wang PS. Involvement of Cdk5/p25 in digoxin-triggered prostate cancer cell apoptosis. J Biol Chem. 2004; 279(28): 29302-7[DOI][PubMed]
  • 4. Semenza GL. Hypoxia-inducible factors: mediators of cancer progression and targets for cancer therapy. Trends Pharmacol Sci. 2012; 33(4): 207-14[DOI][PubMed]
  • 5. Menger L, Vacchelli E, Kepp O, Eggermont A, Tartour E, Zitvogel L, et al. Trial watch: Cardiac glycosides and cancer therapy. Oncoimmunology. 2013; 2(2)[DOI][PubMed]
  • 6. Colditz GA, Hankinson SE, Hunter DJ, Willett WC, Manson JE, Stampfer MJ, et al. The use of estrogens and progestins and the risk of breast cancer in postmenopausal women. N Engl J Med. 1995; 332(24): 1589-93[DOI][PubMed]
  • 7. Boursi B, Haynes K, Mamtani R, Yang YX. Digoxin use and the risk for colorectal cancer. Pharmacoepidemiol Drug Saf. 2014; 23(11): 1147-53[DOI][PubMed]
  • 8. Ahern TP, Tamimi RM, Rosner BA, Hankinson SE. Digoxin use and risk of invasive breast cancer: evidence from the Nurses' Health Study and meta-analysis. Breast Cancer Res Treat. 2014; 144(2): 427-35[DOI][PubMed]
  • 9. Flahavan EM, Sharp L, Bennett K, Barron TI. A cohort study of digoxin exposure and mortality in men with prostate cancer. BJU Int. 2014; 113(2): 236-45[DOI][PubMed]
  • 10. Lu GY, Liu ST, Huang SM, Chang YL, Lin WS. Multiple effects of digoxin on subsets of cancer-associated genes through the alternative splicing pathway. Biochimie. 2014; 106: 131-9[DOI][PubMed]
  • 11. Campisi J. Senescent cells, tumor suppression, and organismal aging: good citizens, bad neighbors. Cell. 2005; 120(4): 513-22[DOI][PubMed]
  • 12. Collado M, Blasco MA, Serrano M. Cellular senescence in cancer and aging. Cell. 2007; 130(2): 223-33[DOI][PubMed]
  • 13. DiDomenico RJ, Bress AP, Na-Thalang K, Tsao YY, Groo VL, Deyo KL, et al. Use of a simplified nomogram to individualize digoxin dosing versus standard dosing practices in patients with heart failure. Pharmacotherapy. 2014; 34(11): 1121-31[DOI][PubMed]
  • 14. Dimas K, Papadopoulou N, Baskakis C, Prousis KC, Tsakos M, Alkahtani S, et al. Steroidal cardiac Na+/K+ ATPase inhibitors exhibit strong anti-cancer potential in vitro and in prostate and lung cancer xenografts in vivo. Anticancer Agents Med Chem. 2014; 14(5): 762-70[DOI][PubMed]
  • 15. Riganti C, Campia I, Polimeni M, Pescarmona G, Ghigo D, Bosia A. Digoxin and ouabain induce P-glycoprotein by activating calmodulin kinase II and hypoxia-inducible factor-1alpha in human colon cancer cells. Toxicol Appl Pharmacol. 2009; 240(3): 385-92[DOI][PubMed]
  • 16. Zhao Y, Zhu C, Li X, Zhang Z, Yuan Y, Ni Y, et al. Asterosaponin 1 induces endoplasmic reticulum stress-associated apoptosis in A549 human lung cancer cells. Oncol Rep. 2011; 26(4): 919-24[DOI][PubMed]
  • 17. Nasri HR, Shahouzehi B, Masoumi-Ardakani Y, Iranpour M. Effects of digoxin on cardiac iron content in rat model of iron overload. ARYA Atheroscler. 2016; 12(4): 180-4[PubMed]
  • 18. Winnicka K, Bielawski K, Bielawska A. Cardiac glycosides in cancer research and cancer therapy. Acta Pol Pharm. 2006; 63(2): 109-15[PubMed]
  • 19. Lopez-Lazaro M, Pastor N, Azrak SS, Ayuso MJ, Austin CA, Cortes F. Digitoxin inhibits the growth of cancer cell lines at concentrations commonly found in cardiac patients. J Nat Prod. 2005; 68(11): 1642-5[DOI][PubMed]
  • 20. Xu ZW, Wang FM, Gao MJ, Chen XY, Hu WL, Xu RC. Targeting the Na(+)/K(+)-ATPase alpha1 subunit of hepatoma HepG2 cell line to induce apoptosis and cell cycle arresting. Biol Pharm Bull. 2010; 33(5): 743-51[DOI][PubMed]
  • 21. Babula P, Masarik M, Adam V, Provaznik I, Kizek R. From Na+/K+-ATPase and cardiac glycosides to cytotoxicity and cancer treatment. Anticancer Agents Med Chem. 2013; 13(7): 1069-87[PubMed]
  • 22. Semenza GL. HIF-1 and tumor progression: pathophysiology and therapeutics. Trends Mol Med. 2002; 8(4 Suppl)-7[PubMed]
  • 23. Gilkes DM, Semenza GL. Role of hypoxia-inducible factors in breast cancer metastasis. Future Oncol. 2013; 9(11): 1623-36[DOI][PubMed]
  • 24. Biggar RJ, Wohlfahrt J, Melbye M. Digoxin use and the risk of cancers of the corpus uteri, ovary and cervix. Int J Cancer. 2012; 131(3): 716-21[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments