Gene, Cell and Tissue

Published by: Kowsar

Withania coagulans Extract Attenuates Histopathological Alteration and Apoptosis in Rat Brain Cortex Following Ischemia/Reperfusion Injury

Maryam Sarbishegi 1 , 2 , Zahra Heidari 3 , 4 , * and Hamidreza Mahmoudzadeh Sagheb 3 , 4
Authors Information
1 Cellular and Molecular Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran
2 Department of Anatomy, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IR Iran
3 Infection Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, IR Iran
4 Department of Histology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, IR Iran
Article information
  • Gene, Cell and Tissue: January 01, 2016, 3 (1); e35295
  • Published Online: January 27, 2016
  • Article Type: Research Article
  • Received: December 6, 2015
  • Revised: January 5, 2016
  • Accepted: January 5, 2016
  • DOI: 10.17795/gct-35295

To Cite: Sarbishegi M, Heidari Z, Mahmoudzadeh Sagheb H. Withania coagulans Extract Attenuates Histopathological Alteration and Apoptosis in Rat Brain Cortex Following Ischemia/Reperfusion Injury, Gene Cell Tissue. 2016 ; 3(1):e35295. doi: 10.17795/gct-35295.

Copyright © 2016, Zahedan University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Chacon MR, Jensen MB, Sattin JA, Zivin JA. Neuroprotection in cerebral ischemia: emphasis on the SAINT trial. Curr Cardiol Rep. 2008; 10(1): 37-42[PubMed]
  • 2. Mahajan SK, Kashyap R, Sood BR, Jaret P, Mokta J, Kaushik NK, et al. Stroke at moderate altitude. J Assoc Physicians India. 2004; 52: 699-702[PubMed]
  • 3. Janardhan V, Qureshi AI. Mechanisms of ischemic brain injury. Curr Cardiol Rep. 2004; 6(2): 117-23[PubMed]
  • 4. Pasban-Aliabadi H, Esmaeili-Mahani S, Sheibani V, Abbasnejad M, Mehdizadeh A, Yaghoobi MM. Inhibition of 6-hydroxydopamine-induced PC12 cell apoptosis by olive (Olea europaea L.) leaf extract is performed by its main component oleuropein. Rejuvenation Res. 2013; 16(2): 134-42[DOI][PubMed]
  • 5. Durairajan SS, Liu LF, Lu JH, Chen LL, Yuan Q, Chung SK, et al. Berberine ameliorates beta-amyloid pathology, gliosis, and cognitive impairment in an Alzheimer's disease transgenic mouse model. Neurobiol Aging. 2012; 33(12): 2903-19[DOI][PubMed]
  • 6. Zhao J, Yu S, Zheng W, Feng G, Luo G, Wang L, et al. Curcumin improves outcomes and attenuates focal cerebral ischemic injury via antiapoptotic mechanisms in rats. Neurochem Res. 2010; 35(3): 374-9[DOI][PubMed]
  • 7. Beigomi M, Mohammadifar MA, Hashemi M, rohani MG, Senthil K, Valizadeh M. Biochemical and rheological characterization of a protease from fruits of Withania coagulans with a milk-clotting activity. Food Sci Biotechnol. 2014; 23(6): 1805-13[DOI]
  • 8. Khodaei M, Jafari M, Noori M. Remedial use of withanolides from Withania coagolans (Stocks) Dunal. Adv Life Sci. 2012; 2(1): 6-19[DOI]
  • 9. Kaileh M, Vanden Berghe W, Heyerick A, Horion J, Piette J, Libert C, et al. Withaferin a strongly elicits IkappaB kinase beta hyperphosphorylation concomitant with potent inhibition of its kinase activity. J Biol Chem. 2007; 282(7): 4253-64[DOI][PubMed]
  • 10. Wube AA, Wenzig EM, Gibbons S, Asres K, Bauer R, Bucar F. Constituents of the stem bark of Discopodium penninervium and their LTB4 and COX-1 and -2 inhibitory activities. Phytochemistry. 2008; 69(4): 982-7[DOI][PubMed]
  • 11. Patil SP, Maki S, Khedkar SA, Rigby AC, Chan C. Withanolide A and asiatic acid modulate multiple targets associated with amyloid-beta precursor protein processing and amyloid-beta protein clearance. J Nat Prod. 2010; 73(7): 1196-202[DOI][PubMed]
  • 12. Choudhary MI, Nawaz SA, ul-Haq Z, Lodhi MA, Ghayur MN, Jalil S, et al. Withanolides, a new class of natural cholinesterase inhibitors with calcium antagonistic properties. Biochem Biophys Res Commun. 2005; 334(1): 276-87[PubMed]
  • 13. Mohanty I, Arya DS, Dinda A, Talwar KK, Joshi S, Gupta SK. Mechanisms of cardioprotective effect of Withania somnifera in experimentally induced myocardial infarction. Basic Clin Pharmacol Toxicol. 2004; 94(4): 184-90[DOI][PubMed]
  • 14. Jain S, Shukla SD, Sharma K, Bhatnagar M. Neuroprotective effects of Withania somnifera Dunn. in hippocampal sub-regions of female albino rat. Phytother Res. 2001; 15(6): 544-8[PubMed]
  • 15. Yigitkanli K, Pekcec A, Karatas H, Pallast S, Mandeville E, Joshi N, et al. Inhibition of 12/15-lipoxygenase as therapeutic strategy to treat stroke. Ann Neurol. 2013; 73(1): 129-35[DOI][PubMed]
  • 16. Sarshoori JR, Asadi MH, Mohammadi MT. Neuroprotective effects of crocin on the histopathological alterations following brain ischemia-reperfusion injury in rat. Iran J Basic Med Sci. 2014; 17(11): 895-902[PubMed]
  • 17. Chen H, Yoshioka H, Kim GS, Jung JE, Okami N, Sakata H, et al. Oxidative stress in ischemic brain damage: mechanisms of cell death and potential molecular targets for neuroprotection. Antioxid Redox Signal. 2011; 14(8): 1505-17[DOI][PubMed]
  • 18. Gaur V, Aggarwal A, Kumar A. Protective effect of naringin against ischemic reperfusion cerebral injury: possible neurobehavioral, biochemical and cellular alterations in rat brain. Eur J Pharmacol. 2009; 616(1-3): 147-54[DOI][PubMed]
  • 19. Margaritescu O, Mogoanta L, Pirici I, Pirici D, Cernea D, Margaritescu C. Histopathological changes in acute ischemic stroke. Rom J Morphol Embryol. 2009; 50(3): 327-39[PubMed]
  • 20. Chandrashekhar VM, Ranpariya VL, Ganapaty S, Parashar A, Muchandi AA. Neuroprotective activity of Matricaria recutita Linn against global model of ischemia in rats. J Ethnopharmacol. 2010; 127(3): 645-51[DOI][PubMed]
  • 21. Zhou XQ, Zeng XN, Kong H, Sun XL. Neuroprotective effects of berberine on stroke models in vitro and in vivo. Neurosci Lett. 2008; 447(1): 31-6[DOI][PubMed]
  • 22. Shrivastava V, Mathur D, Agrawal RC. Phytochemical Screening and Determination of Antioxidant Potential of Fruits Extracts of Withania coagulans. Recent Res Sci Technol. 2011; 3(11)
  • 23. Prasad SK, Kumar R, Patel DK, Hemalatha S. Wound healing activity of Withania coagulans in streptozotocin-induced diabetic rats. Pharm Biol. 2010; 48(12): 1397-404[DOI][PubMed]
  • 24. Sarbishegi M, Khani M, Salimi S, Valizadeh M, Sargolzaei Aval F. Antiproliferative and Antioxidant Effects of Withania coagulans Extract on Benign Prostatic Hyperplasia in Rats. Nephro Urol Mon. 2016; 8(1)[DOI]
  • 25. Jha S, Calvert JW, Duranski MR, Ramachandran A, Lefer DJ. Hydrogen sulfide attenuates hepatic ischemia-reperfusion injury: role of antioxidant and antiapoptotic signaling. Am J Physiol Heart Circ Physiol. 2008; 295(2)-6[DOI][PubMed]
  • 26. Gilgun-Sherki Y, Rosenbaum Z, Melamed E, Offen D. Antioxidant therapy in acute central nervous system injury: current state. Pharmacol Rev. 2002; 54(2): 271-84[PubMed]
  • 27. Sarbishegi M, Heidari Z, Mahmoudzadeh-Sagheb H, Valizadeh M, Doostkami M. Neuroprotective effects of Withania coagulans root extract on CA1 hippocamus following cerebral ischemia in rats. Avicenna J Phytomed. 2016; : 1-11
  • 28. Budhiraja RD, Garg KN, Sudhir S, Arora B. Protective effect of 3-beta-hydroxy-2,3-dihydrowithanolide F against CCl4-induced hepatotoxicity. Planta Med. 1986; (1): 28-9[DOI][PubMed]
  • 29. Oh JH, Kwon TK. Withaferin A inhibits tumor necrosis factor-alpha-induced expression of cell adhesion molecules by inactivation of Akt and NF-kappaB in human pulmonary epithelial cells. Int Immunopharmacol. 2009; 9(5): 614-9[DOI][PubMed]
  • 30. Lalsare S, Chutervedi A. Anti-inflammatory and anthihyperlipidemic activity of various extract of fruits Withania coagulance. Pharmacologyonline. 2010; 1: 101-7
  • 31. Bemeur C, Qu H, Desjardins P, Butterworth RF. IL-1 or TNF receptor gene deletion delays onset of encephalopathy and attenuates brain edema in experimental acute liver failure. Neurochem Int. 2010; 56(2): 213-5[DOI][PubMed]
  • 32. Webster CM, Kelly S, Koike MA, Chock VY, Giffard RG, Yenari MA. Inflammation and NFkappaB activation is decreased by hypothermia following global cerebral ischemia. Neurobiol Dis. 2009; 33(2): 301-12[DOI][PubMed]
  • 33. Capone C, Fabrizi C, Piovesan P, Principato MC, Marzorati P, Ghirardi O, et al. 2-Aminotetraline derivative protects from ischemia/reperfusion brain injury with a broad therapeutic window. Neuropsychopharmacology. 2007; 32(6): 1302-11[DOI][PubMed]
  • 34. Bhatnagar M, Meena P, Barbar S, Joshi C. Neuroprotective response of the hippocampus region of the brain to Withania somnifera and Asparagus racemosus root extract: An in vitro study. Afr J Zool. 2013; 2010: 1
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments