Gene, Cell and Tissue

Published by: Kowsar

Standardization of Molecular Diagnostic of the entC and ent E Staphylococcus aureus Isolated from Human Infections in Zabol

Khadije Rezaie Keikhaie 1 , Gholamreza Bagheri 1 , Atefeh Kamali 2 , Saphora Bazi 3 , Zahra Shahi 2 and Fereshteh Javadian 4 , *
Authors Information
1 Obstetrics and Gynecology Dept., Maternal and Fetal Health Research Center, Zabol University of Medical Sciences, Zabol, Iran
2 Department of Microbiology, Islamic Azad University, Kerman Branch, Kerman, Iran
3 Payame Noor University, Zabol, Iran
4 Zabol Medicinal Plant Research Center, Zabol University of Medical Sciences, Zabol, Iran
Article information
  • Gene, Cell and Tissue: October 2017, 4 (4); e59214
  • Published Online: October 31, 2017
  • Article Type: Research Article
  • Received: August 5, 2017
  • Revised: September 10, 2017
  • Accepted: October 20, 2017
  • DOI: 10.5812/gct.59214

To Cite: Rezaie Keikhaie K, Bagheri G, Kamali A, Bazi S, Shahi Z, et al. Standardization of Molecular Diagnostic of the entC and ent E Staphylococcus aureus Isolated from Human Infections in Zabol, Gene Cell Tissue. 2017 ; 4(4):e59214. doi: 10.5812/gct.59214.

Abstract
Copyright © 2017, Gene, Cell and Tissue. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited
1. Background
2. Methods
3. Results
4. Discussion
Acknowledgements
Footnotes
References
  • 1. Kilic A, Basustaoglu AC. Double triplex real-time PCR assay for simultaneous detection of Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus hominis, and Staphylococcus haemolyticus and determination of their methicillin resistance directly from positive blood culture bottles. Res Microbiol. 2011;162(10):1060-6. doi: 10.1016/j.resmic.2011.07.009. [PubMed: 21925597].
  • 2. Hu DL, Maina EK, Omoe K, Inoue F, Yasujima M, Nakane A. Superantigenic toxin genes coexist with specific staphylococcal cassette chromosome mec genes in methicillin-resistant Staphylococcus aureus. Tohoku J Exp Med. 2011;225(3):161-9. doi: 10.1620/tjem.225.161. [PubMed: 21971303].
  • 3. Dinges MM, Orwin PM, Schlievert PM. Exotoxins of Staphylococcus aureus. Clin Microbiol Rev. 2000;13(1):16-34. table of contents. doi: 10.1128/CMR.13.1.16-34.2000. [PubMed: 10627489].
  • 4. Parsonnet J, Hansmann MA, Delaney ML, Modern PA, Dubois AM, Wieland-Alter W, et al. Prevalence of toxic shock syndrome toxin 1-producing Staphylococcus aureus and the presence of antibodies to this superantigen in menstruating women. J Clin Microbiol. 2005;43(9):4628-34. doi: 10.1128/JCM.43.9.4628-4634.2005. [PubMed: 16145118].
  • 5. Lowy FD. Staphylococcus aureus infections. N Engl J Med. 1998;339(8):520-32. doi: 10.1056/NEJM199808203390806. [PubMed: 9709046].
  • 6. Vasconcelos NG, Cunha MR. Staphylococcal enterotoxins: molecular aspects and detection methods. J Public Health Epidemiol. 2010;2(29-42).
  • 7. Abe J, Ito Y, Onimaru M, Kohsaka T, Takeda T. Characterization and distribution of a new enterotoxin-related superantigen produced by Staphylococcus aureus. Microbiol Immunol. 2000;44(2):79-88. doi: 10.1111/j.1348-0421.2000.tb01250.x. [PubMed: 10803494].
  • 8. Shuiep ES, Kanbar T, Eissa N, Alber J, Lammler C, Zschock M, et al. Phenotypic and genotypic characterization of Staphylococcus aureus isolated from raw camel milk samples. Res Vet Sci. 2009;86(2):211-5. doi: 10.1016/j.rvsc.2008.07.011. [PubMed: 18778841].
  • 9. Omoe K, Hu DL, Takahashi-Omoe H, Nakane A, Shinagawa K. Comprehensive analysis of classical and newly described staphylococcal superantigenic toxin genes in Staphylococcus aureus isolates. FEMS Microbiol Lett. 2005;246(2):191-8. doi: 10.1016/j.femsle.2005.04.007. [PubMed: 15899405].
  • 10. van Belkum A. Molecular diagnostics in medical microbiology: yesterday, today and tomorrow. Curr Opin Pharmacol. 2003;3(5):497-501. doi: 10.1016/S1471-4892(03)00108-5. [PubMed: 14559094].
  • 11. Eshraghi S, Salehipour Z, Pourmand MR, Rahimi Forushani A, Zahraei Salehi MT, Agha Amiri S. Prevalence of tst, entC, entA and entA/C genes in Staphylococcus aureus strains isolated from different foods. Tehran Univ Med J. 2009;67:470-6.
  • 12. Chapaval L, Moon DH, Gomes JE, Duarte FR, Tsai SM. Use of PCR to detect classical enterotoxins genes (ENT) and toxic shock syndrome toxin-1 gene (TST) in Staphylococcus aureus isolated from crude milk and determination of toxin productivities of S. aureus isolateds harboring these genes. Arq Inst Biol. 2006;73:165-9.
  • 13. Chiang YC, Liao WW, Fan CM, Pai WY, Chiou CS, Tsen HY. PCR detection of Staphylococcal enterotoxins (SEs) N, O, P, Q, R, U, and survey of SE types in Staphylococcus aureus isolates from food-poisoning cases in Taiwan. Int J Food Microbiol. 2008;121(1):66-73. doi: 10.1016/j.ijfoodmicro.2007.10.005. [PubMed: 18068843].
  • 14. Bayles KW, Iandolo JJ. Genetic and molecular analyses of the gene encoding staphylococcal enterotoxin D. J Bacteriol. 1989;171(9):4799-806. doi: 10.1128/jb.171.9.4799-4806.1989. [PubMed: 2549000].
  • 15. Giezendanner N, Meyer B, Gort M, Muller P, Zweifel C. [Raw milk-associated Staphylococcus aureus intoxication in children]. Schweiz Arch Tierheilkd. 2009;151(7):329-31. doi: 10.1024/0036-7281.151.7.329. [PubMed: 19565455].
  • 16. Ataee RA, Mehrabi-Tavana A, Izadi M, Hosseini SM, Ataee MH. Bacterial meningitis: a new risk factor. J Res Med Sci. 2011;16(2):207-10. [PubMed: 22091233].
  • 17. Clinical and Laboratory standards institute . MO 7-A9: Methods for dilution antimicrobial susceptibility test for bacteria that grow aerobically approved standard MO7- A9. 32. 9th ed. 2012.
  • 18. Balaban N, Rasooly A. Staphylococcal enterotoxins. Int J Food Microbiol. 2000;61(1):1-10. doi: 10.1016/S0168-1605(00)00377-9. [PubMed: 11028954].
  • 19. Ataee RA, Kahani MS, Alishiri GH, Ahamadi Z. Staphylococcal Enterotoxin A Detection from Rheumatoid Arthritis Patients' Blood and Synovial Fluid. Electron Physician. 2016;8(2):1850-6. doi: 10.19082/1850. [PubMed: 27053990].
  • 20. Ataee RA, Kashefi R, Alishiri GH, Esmaieli D. Assay of Blood and Synovial Fluid of Patients With Rheumatoid Arthritis for Staphylococcus aureus Enterotoxin D: Absence of Bacteria But Presence of Its Toxin. Jundishapur J Microbiol. 2015;8(12). e28395. doi: 10.5812/jjm.28395. [PubMed: 26870313].
  • 21. Noorbakhsh S, Talebi-Taher M, Tabatabaei A. Identification of bacterial antigens and super antigens in synovial fluid of patients with arthritis: a cross sectional study. Med J Islam Repub Iran. 2013;27(1):12-6. [PubMed: 23483360].
  • 22. Vali Zadeh A, Amini K. Staphylococcus aureus enterotoxin genes detected by Multiplex PCR [In Persian]. J Babol Univ Med Sci. 2016;18(12):26-32.
  • 23. Saadati M, Barati B, Shirazi M. Identification of Sea, Sec and Seq genes of Staphylococcus aureus from healthy specimens. Biannual J South Med. 2009;12(1):8-16.
  • 24. Norouzi J, Goudarzi G, Pakzad P, Razavipour R. Isolation and detection of Staphylococcus aureus enterotoxins A-E and TSSt-1 genes from different sources by PCR method [In Persian]. Qom Univ Med Sci J. 2012;6(3):78-85.
  • 25. Zahiri Yeganeh S, Ataee RA, Alishiri GH, Movahedi M. Bacteriological and molecular assessment of staphylococcal enterotoxin e in the blood of patients with rheumatoid arthritis. Jundishapur J Microbiol. 2015;8(2). e16621. doi: 10.5812/jjm.16621. [PubMed: 25793096].
  • 26. Noorbakhsh S, Farhadi M, Tabatabaei A. Staphylococcus super antigens, Toxic shock syndrom toxin-1 and enterotoxin in pediatric otitis media effusion: A brief report [In Persian]. Tehran Univ Med J. 2013;70(12):793-7.
  • 27. Ataee RA, Kamali M, Ranjbar R, Karami A, Ghorbani M. Standardization of molecular diagnostic of the entC staphylococcus aureus isolated from human infections and determine its sequence. Iran J Mil Med. 2012;14(4):226-35.
  • 28. Bokaeian M, Tahmasebi H, Shahraki Zahedani SH, Abadi J. An investigation of toxic shock syndrom Toxin- 1 Gene in methicillin resistant clinical strains of Staphylococcus aureus using multiplex- PCR method [In Persian]. Qom Univ Med Sci J. 2017;11(1):57-67.
  • 29. Vaez H, Ghazi Saeidi K, Moradi A, Tabaraei A, Khodabakhshi B, Bazouri M, et al. Antibiotic resistance pattern of methicillin resistant Staphylococcus aureus isolated from Health-educational centers of Gorgan, Iran, 2008-2009. Iran J Microbiol. 2009;3(4):31-6.
  • 30. Parhizgari N, Moosavian M, Sharifi A. Antibiotic resistant pattern of methicillin resistant and sensitive Staphylococcus aureus isolated from patients durining 2009-2010, Ahvaz, Iran [In Persian]. Armaghan-e-Danesh Yasuj Univ Med Sci J. 2013;18(9).
  • 31. Mousae S, Fateh R, Eshaghi E, Khalifeh-Gholi M. Investigation of antibiotic susceptibility pattern of Staphylococcus aureus strains isolated from patients referring to some treatment centers of Qom City, Iran [In Persian]. Qom Univ Med Sci J. 2017;11(5):98-106.
  • 32. Moradi N, Javadpour S, Karmostaji A. Reduced sensitivity of staphylococcus aureus to vancomycin [In Persian]. Hormozgan Univ Med Sci. 2011;15(3).
  • 33. Faraj Zadeh Sheikh A, Ranjbar Y, Meghdadi H, Alami A. The frequency of enterotoxin a genes and its association with antimicrobial resistance among Staphylococcus aureus isolates from clinical specimens from patients admitted to Golestan and Imam Khomeini hospitals in Ahvaz, Iran [In Persian]. Jundishapur Sci Med J. 2015;14(3):301-8.
  • 34. Tafaroji J, Aghaali M, Heydari H. An investigation of the frequency of staphylococcus aureus nasal carriers and its antibiotic susceptibility pattern in the staff of different wards of Qom Hazrat Masumeh hospital, 2015, Iran [In Persian]. Qom Univ Med Sci J. 2017;10(11):79-84.
  • 35. Mahdiyoun SM, Ahanjan M, Goudarzi M, Rezaee R. Prevalence of antibiotic resistance in methicillin-resistant Staphylococcus aureus and determining aminoglycoside resistance gene by PCR in Sari and Tehran hospitals [In Persian]. J Mazandaran Univ Med Sci. 2015;25(128):97-107.
  • 36. Tabaei S, Kouhi Noghondar M, Mohammadzadeh M, Ataei L, Jamehdar SA. Pattern of antibiotic resistance in methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from clinical specimens: Imam Reza hospital in Mashhad [In Persian]. Mashhad Univ Med Sci. 2016;2(59):64-70.
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