Gene, Cell and Tissue

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Study of Prevalence of Toxic Shock Syndrome Toxin (TSST-1) and Methicilin Resistance (MecA) Genes of Staphylococcus aureus Isolates from Local Cheese in Northwest of Iran

Parvaneh Ghasemi 1 and Saman Mahdavi 2 , *
Authors Information
1 Microbiology Scientific Community, Maragheh Branch, Islamic Azad University, Maragheh, Iran
2 Department of Microbiology, Maragheh Branch, Islamic Azad University, Maragheh, Iran
Article information
  • Gene, Cell and Tissue: 5 (4); e82372
  • Published Online: December 26, 2018
  • Article Type: Research Article
  • Received: July 18, 2018
  • Revised: December 14, 2018
  • Accepted: December 18, 2018
  • DOI: 10.5812/gct.82372

To Cite: Ghasemi P , Mahdavi S. Study of Prevalence of Toxic Shock Syndrome Toxin (TSST-1) and Methicilin Resistance (MecA) Genes of Staphylococcus aureus Isolates from Local Cheese in Northwest of Iran, Gene Cell Tissue. Online ahead of Print ; 5(4):e82372. doi: 10.5812/gct.82372.

Copyright © 2018, Gene, Cell and Tissue. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Methods
4. Results
5. Discussion
  • 1. Quinn PJ, Quinn PJ, Carter ME, Markey B, Carter GR. Clinical veterinary microbiology. 2nd ed. New York: Wolfe; 1994.
  • 2. Bennett RW, Monday SR. Staphylococcus aureus. In: Miliotis MD, Bier JW, editors. International handbook of foodborne pathogens. New York: Marcel Dekker, Inc; 2003.
  • 3. Le Loir Y, Baron F, Gautier M. Staphylococcus aureus and food poisoning. Genet Mol Res. 2003;2(1):63-76. [PubMed: 12917803].
  • 4. Wieneke AA, Roberts D, Gilbert RJ. Staphylococcal food poisoning in the United Kingdom, 1969-90. Epidemiol Infect. 1993;110(3):519-31. [PubMed: 8519317]. [PubMed Central: PMC2272286].
  • 5. Najafi A, Deylami Ziabakhsh M, Karimian H, Abedinia AR, Hosseininezhad M. [Microbiological changes of pousti cheese during ripening]. Food Technol Nutr. 2011;8(2):85-91. Persian.
  • 6. Salek Moghadam A, Forouhesh Tehrani H, Ansari H, Ravadgar B, Noorani Vatani A, Ghassemi M. [A survey on bacterial contamination on one-hundred unpasteurized cheese samples and pasteurized cheese as control and stability of commonly contaminating bacteria to different salt concentration]. Razi J Med Sci. 2001;8(25):175-81. Persian.
  • 7. Moroni P, Pisoni G, Cremonesi P, Castiglioni B. Staphylococcus. In: Liu D, editor. Molecular detection of foodborne pathogens. CRC Press; 2009.
  • 8. Fueyo JM, Mendoza MC, Rodicio MR, Muniz J, Alvarez MA, Martin MC. Cytotoxin and pyrogenic toxin superantigen gene profiles of Staphylococcus aureus associated with subclinical mastitis in dairy cows and relationships with macrorestriction genomic profiles. J Clin Microbiol. 2005;43(3):1278-84. doi: 10.1128/JCM.43.3.1278-1284.2005. [PubMed: 15750096]. [PubMed Central: PMC1081256].
  • 9. Shimamura Y, Kidokoro S, Murata M. Survey and properties of Staphylococcus aureus isolated from Japanese-style desserts. Biosci Biotechnol Biochem. 2006;70(7):1571-7. doi: 10.1271/bbb.50617. [PubMed: 16819155].
  • 10. Alizadeh S, Amini K. [Determining the presence of virulence genes panton valentine leukocidin pvl and methicillin resistance gene meca in Staphylococcus aureus strains isolated from food samples by multiplex pcr and antibiotic resistance]. J Food Microbiol. 2015;2(4):49-58. Persian.
  • 11. Brakstad OG, Aasbakk K, Maeland JA. Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. J Clin Microbiol. 1992;30(7):1654-60. [PubMed: 1629319]. [PubMed Central: PMC265359].
  • 12. Mehrotra M, Wang G, Johnson WM. Multiplex PCR for detection of genes for Staphylococcus aureus enterotoxins, exfoliative toxins, toxic shock syndrome toxin 1, and methicillin resistance. J Clin Microbiol. 2000;38(3):1032-5. [PubMed: 10698991]. [PubMed Central: PMC86330].
  • 13. Eshraghi S, Salehipour Z, Pourmand MR, Forushani AR, Salehi MT, Agha Amiri S, et al. Prevalence of tst, entC, entA and entA/C genes in staphylococcus aureus strains isolated from different foods. Tehran Univ Med J. 2009;67(7):470-6.
  • 14. Morad Nia H, Mohamadi Sani A, Khezri M. [Study of prevalence of antibiotic resistance to methicillin in Staphylococcus aureus strains isolated from meaty and dairy foods in Mashhad city]. Innov Food Sci Emerg Technol. 2013;6(2):1-9. Persian.
  • 15. Pexara A, Solomakos N, Govaris A. Prevalence of methicillin-resistant Staphylococcus aureus in milk and dairy products. J Hellenic Vet Med Soc. 2017;64(1):17. doi: 10.12681/jhvms.15449.
  • 16. Lowy FD. Antimicrobial resistance: The example of Staphylococcus aureus. J Clin Invest. 2003;111(9):1265-73. doi: 10.1172/JCI18535. [PubMed: 12727914]. [PubMed Central: PMC154455].
  • 17. Arcuri EF, Angelo FF, Guimaraes MF, Talon R, Borges Mde F, Leroy S, et al. Toxigenic status of Staphylococcus aureus isolated from bovine raw milk and Minas frescal cheese in Brazil. J Food Prot. 2010;73(12):2225-31. [PubMed: 21219740].
  • 18. Oh SK, Lee N, Cho YS, Shin DB, Choi SY, Koo M. Occurrence of toxigenic Staphylococcus aureus in ready-to-eat food in Korea. J Food Prot. 2007;70(5):1153-8. [PubMed: 17536673].
  • 19. Zschöck M, Botzler D, Blöcher S, Sommerhäuser J, Hamann HP. Detection of genes for enterotoxins (ent) and toxic shock syndrome toxin-1 (tst) in mammary isolates of Staphylococcus aureus by polymerase-chain-reaction. Int Dairy J. 2000;10(8):569-74. doi: 10.1016/s0958-6946(00)00084-4.
  • 20. Farahmand-Azar S, Ahmadi M, Saei HD, Anassori E. Identification of toxic shock syndrome toxin-1 (TSST-1) gene in Staphylococcus aureus isolated from bovine mastitis milk. Arch Razi Inst. 2013;68(1):17-22.
  • 21. Norouzi J, Goudarzi G, Pakzad P, Razavipour R. [The isolation and detection of Staphylococcus aureus enterotoxins AE and TSST-1 genes from different sources by PCR method]. Qom Univ Med Sci J. 2012;6(3):78-85. Persian.

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